This video demonstrates correct aseptic transfer techniques used in a microbiology laboratory. Students are walked through the procedure used to transfer microbes from both liquid and solid media, to agar slants and broth tubes. We also demonstrate how to perform a streak plate and a pour plate. Results after incubation are also shown.
In this video, we demonstrate how to pour molten agar from individual tubes or large flasks into petri plates. We also highlight the importance of using aseptic technique, and which points are most likely to introduce contamination. This video was prepared for FOOD 1120.
In order to view microbiological specimens under a microscope, it is first necessary to prepare an appropriate microscope slide. In this video, we demonstrate how to prepare and fix a smear onto a glass microscope slide. We then demonstrate how to perform a simple stain with crystal violet, and a very commonly used differential stain (Gram stain).
In this video, we demonstrate how to pour molten agar from individual tubes or large flasks into petri plates. We also highlight the importance of using aseptic technique, and which points are most likely to introduce contamination. This video was prepared for BIOT 3210 and BIOT 3201, and shows the equipment typically used in SW9-209.
In order to view microbiological specimens under a microscope, it is first necessary to prepare an appropriate microscope slide. In this video, we demonstrate how to prepare and fix a smear of a broth culture of microbes onto a glass microscope slide. We then demonstrate how to perform the Gram stain technique, a very commonly used differential stain.
This video introduces the viewer to the parts of a typical compound microscope, and describes the functions of the different parts of the microscope. Proper care and safe handling of the microscope is also discussed.
This video shows how to get a Gram stained slide in focus on a compound microscope. It shows the correct use of a microscope, and the series of steps required to eventually bring a bacterial specimen into focus with the 100x oil immersion lens.
This video demonstrates correct aseptic transfer techniques used in a microbiology laboratory. Students are walked through the procedure used to transfer microbes into agar slants, broth tubes, and agar deeps.
This video shows the pattern used when performing a streak plate. It is demonstrated on a whiteboard in order to give students a clearer image of how to perform the technique.
When enumerating bacteria, it is necessary to dilute your culture in order to obtain a plate with a countable number of microbes. This video shows the steps needed to prepare a dilution series, both with a theoretical demonstration, and a demonstration of the actual lab techniques.
