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Title
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Microbiology Lab Basics: Aseptic transfer techniques
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Date
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2021
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Description
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This video demonstrates correct aseptic transfer techniques used in a microbiology laboratory. Students are walked through the procedure used to transfer microbes from both liquid and solid media, ...
Show moreThis video demonstrates correct aseptic transfer techniques used in a microbiology laboratory. Students are walked through the procedure used to transfer microbes from both liquid and solid media, to agar slants and broth tubes. We also demonstrate how to perform a streak plate and a pour plate. Results after incubation are also shown.
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Microbiology Lab Basics: Preparing and Staining a Smear
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Date
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2021
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Description
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In order to view microbiological specimens under a microscope, it is first necessary to prepare an appropriate microscope slide. In this video, we demonstrate how to prepare and fix a smear onto a ...
Show moreIn order to view microbiological specimens under a microscope, it is first necessary to prepare an appropriate microscope slide. In this video, we demonstrate how to prepare and fix a smear onto a glass microscope slide. We then demonstrate how to perform a simple stain with crystal violet, and a very commonly used differential stain (Gram stain).
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Microbiology Lab Basics: Pouring agar plates
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Date
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2021
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Description
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In this video, we demonstrate how to pour molten agar from individual tubes or large flasks into petri plates. We also highlight the importance of using aseptic technique, and which points are most...
Show moreIn this video, we demonstrate how to pour molten agar from individual tubes or large flasks into petri plates. We also highlight the importance of using aseptic technique, and which points are most likely to introduce contamination. This video was prepared for FOOD 1120.
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Microbiology Lab Basics: Using an Autoclave
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Date
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2021
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Description
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In this video, we demonstrate how to prepare items for sterilization in an autoclave. We also demonstrate safe usage of a typical large autoclave.
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Video 1 - Pouring Agar Plates
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Date
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2022
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Description
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In this video, we demonstrate how to pour molten agar from individual tubes or large flasks into petri plates. We also highlight the importance of using aseptic technique, and which points are most...
Show moreIn this video, we demonstrate how to pour molten agar from individual tubes or large flasks into petri plates. We also highlight the importance of using aseptic technique, and which points are most likely to introduce contamination. This video was prepared for BIOT 3210 and BIOT 3201, and shows the equipment typically used in SW9-209.
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Video 10 - Preparing a gram stain
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Date
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2022
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Description
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In order to view microbiological specimens under a microscope, it is first necessary to prepare an appropriate microscope slide. In this video, we demonstrate how to prepare and fix a smear of a br...
Show moreIn order to view microbiological specimens under a microscope, it is first necessary to prepare an appropriate microscope slide. In this video, we demonstrate how to prepare and fix a smear of a broth culture of microbes onto a glass microscope slide. We then demonstrate how to perform the Gram stain technique, a very commonly used differential stain.
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Video 7 - Preparing spread plates
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Date
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2022
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Description
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This video demonstrates the spread plate technique, a common microbiological technique for enumeration of aerobic bacteria. This video shows inoculation from a broth culture, but an unknown liquid ...
Show moreThis video demonstrates the spread plate technique, a common microbiological technique for enumeration of aerobic bacteria. This video shows inoculation from a broth culture, but an unknown liquid sample can also be used as your initial inoculum. Our laboratory uses metal spreaders, but glass spreaders can also be used in the same manner.
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Video 5 - Preparing a dilution series
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Date
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2022
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Description
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When enumerating bacteria, it is necessary to dilute your culture in order to obtain a plate with a countable number of microbes. This video shows the steps needed to prepare a dilution series, bot...
Show moreWhen enumerating bacteria, it is necessary to dilute your culture in order to obtain a plate with a countable number of microbes. This video shows the steps needed to prepare a dilution series, both with a theoretical demonstration, and a demonstration of the actual lab techniques.
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Video 12 - Getting your cells in focus
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Date
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2022
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Description
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This video shows how to get a Gram stained slide in focus on a compound microscope. It shows the correct use of a microscope, and the series of steps required to eventually bring a bacterial specim...
Show moreThis video shows how to get a Gram stained slide in focus on a compound microscope. It shows the correct use of a microscope, and the series of steps required to eventually bring a bacterial specimen into focus with the 100x oil immersion lens.
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Video 8 - Results of tube inoculation
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Date
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2022
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Description
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This video shows a set of expected results for after inoculation of broth, slants, and agar deeps, as well as streak plates, pour plates and spread plates. These would be the expected results from ...
Show moreThis video shows a set of expected results for after inoculation of broth, slants, and agar deeps, as well as streak plates, pour plates and spread plates. These would be the expected results from video 2, 4, 6 and 7.
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Title
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Video 2 - Inoculating broths, slants, deeps
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Date
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2022
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Description
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This video demonstrates correct aseptic transfer techniques used in a microbiology laboratory. Students are walked through the procedure used to transfer microbes into agar slants, broth tubes, and...
Show moreThis video demonstrates correct aseptic transfer techniques used in a microbiology laboratory. Students are walked through the procedure used to transfer microbes into agar slants, broth tubes, and agar deeps.
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Video 11 - Microscope orientation
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Date
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2022
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Description
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This video introduces the viewer to the parts of a typical compound microscope, and describes the functions of the different parts of the microscope. Proper care and safe handling of the microscope...
Show moreThis video introduces the viewer to the parts of a typical compound microscope, and describes the functions of the different parts of the microscope. Proper care and safe handling of the microscope is also discussed.
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Video 3 - Whiteboard demo of a streak plate
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Date
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2022
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Description
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This video shows the pattern used when performing a streak plate. It is demonstrated on a whiteboard in order to give students a clearer image of how to perform the technique.
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Title
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Video 4 - Preparing streak plates
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Date
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2022
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Description
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This video demonstrates how to perform one variation of the streak plate technique for obtaining isolated colonies on solid agar plates.
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Video 9 - Dealing with a spill
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Date
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2022
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Description
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This video shows the correct technique for cleaning up a minor biohazardous spill, using a liquid benchtop sanitizer.
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Title
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Differential and Selective Media Assignment Video
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Date
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2021
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Description
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This video is a virtual investigation into the use of differential and selective media as a tool to identify the types of bacteria found in spoiled food samples. We use a serial dilution and spread...
Show moreThis video is a virtual investigation into the use of differential and selective media as a tool to identify the types of bacteria found in spoiled food samples. We use a serial dilution and spread plate technique to inoculate our food samples onto 5 different types of media. We then view those results and compare them to the results expected from certain known bacteria. We have also submitted and accompanying .word document of an assignment that students can complete after watching this video.
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