Environmental Public Health Journal 2016 | BCIT Institutional Repository

Environmental Public Health Journal 2016

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Examining the safety of duck breast prepared the sous vide method
Objectives: There is an increasing desire in the culinary industry to use sous vide to prepare meals at low internal temperatures to enhance flavour, texture, and quality. The sous vide method uses specific time and temperature combinations to allow for sufficient microbial destruction. The BCCDC’s Guidelines for Restaurant Sous Vide Cooking Safety in British Columbia suggests time and temperature combinations to help ensure that the required log10 reductions of pathogens are achieved. Concerns for public safety arise when chefs deviate from the guidelines, and therefore may not achieve the appropriate log10 reductions. This study looked at a commonly used sous vide duck breast recipe and determine whether appropriate the appropriate log10 reductions were met. It also examine the efficacy of the sear step and resting period in achieving the log10 reductions. Methods: After calibration, two batches of 15 duck breasts were prepared using the sous vide method for 80 minutes at 58ºC, the breasts were then seared on a 200°C frying pan for 2 minutes each side, and then subjected to a 4 minute rest period at room temperature. The internal temperature of the breasts was continuously measured using SmartButton thermometers. This data was entered into the AMI Process Lethality Determination Spreadsheet to calculate the log10 reductions. The log10 reductions were analyzed using a one-sample t-test to assess whether the recipe achieved the required 7.0 log10 reductions. Results: The results showed 14% of the 29 duck breasts achieved a 7.0 log10 reduction after the sous vide step of 80 minutes at 58 ºC. The null hypothesis (Ho: measured log10 reductions of duck breasts = 7.0 log10 reductions) was rejected with 100% power and a p-value of 0.00. The mean was 5.13, therefore it seems as though the log10 reductions were significantly lower than 7.0 log10 reductions. After the sear and the resting period, 52% of 27 duck breasts achieved a 7.0 log10 reduction. Statistical analyses showed that the null hypothesis could not be rejected. The p-value was 0.97 and the power was 0.413. Disregarding cumulative effects, the median log10 reductions achieved only by the sear step was 0.43, and the median log10 reductions achieved solely by the resting period was 0.35. Conclusion: Due to lack of normality one cannot confidently say this recipe will achieve 7.0 log10 reductions. However, due to the high log10 reductions achieved, it seems plausible for another recipe to provide adequate log10 reductions while maintaining acceptable quality. The sous vide step should be used for the majority of the log10 reductions. Due to a wide variability in the results, the sear and resting period should only be used for small increases in log10 reductions., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2017., Peer reviewed, Sous vide, Duck breast, Temperature, SmartButton, Public health, Sear, Foodborne illness
An investigation of the effect of awareness and knowledge on emergency planning for food service establishment operators
INTRODUCTION: Current legislative deficit could leave food service establishments without sufficient food safety plans allowing food safety standards to decline during emergencies and increasing risk for foodborne illness. Relevant research indicates there is likely to be a lack of planning and action and lack of accessible resources on the behalf of operators and governmental agencies respectively (Story, 2007). OBJECTIVES: The primary objective of this research was to elucidate the relationship between the dependent variable: food service establishment operator’s level of emergency planning and two independent variables: their level of knowledge and level of awareness. METHODS: A list of suitable candidates was generated using Yellowpages and potential candidates were randomly selected by numerical draw. These individuals were surveyed in person and this process was repeated until 30 contacts were acquired. The data was compiled in Excel for differential statistical analyses and SAS for Chi-squared testing for statistical significance. RESULTS: Chi-square testing indicated a p-value of >0.05 for both data sets; therefore, no association was found between both food operator’s knowledge and awareness and their level of emergency planning and both null hypotheses were rejected. Mean, median, and mode values were determined to fall within the neutral-high value for the ordinal scale. CONCLUSION: Neither awareness and knowledge had a statistically significant relationship to the level of the operator’s level of emergency planning. All tested categories were of neutral-high score values meaning the surveyed data did not find a deficiency in operator planning. These results could be indicative of error in survey design meaning further research on this topic will be necessary to determine a conclusive relationship between these variables., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2016., Peer reviewed, Food operators, Emergency planning, Awareness, Knowledge
Potential environmental impact of the Fukushima Daiichi nuclear plant accident
Background and Purpose: The 2011 Fukushima Daiichi nuclear reactor incident in Japan resulted in the release of large quantities of radioactive material into the Pacific Ocean through deposition from the atmosphere and liquid discharges from the nuclear facility. Public misconceptions and the scarcity of radiation monitoring along the west coast of Canada have create doubt within the population regarding the safety of the ocean. A radiation survey along some of Vancouver’s beaches was completed to determine whether radioactivity from Fukushima has reached our local shores. Methods: Radiation surveys were conducted at three different locations; Sunset, Spanish Banks and Jericho Beach in Vancouver, British Columbia. Gamma radiation levels were measured using both the Radeye, GR 135 Plus EXPLORANIUM survey meters. At each beach, 30 samples of water, sand and various different artifacts including algae, stones and logs were each surveying. If the radiation detected exceed 0.05μSv/Hr the identification function of the GR 135 Plus EXPLORANIUM would be used to determine if it was from natural or artificial sources. The radiation levels were compared to the expected normal background levels (between 0.05μSv/Hr- 1.0 μSv/Hr) as well as to observed if there was any sufficient differences between the mediums under investigation. Results: The radiation levels along all three beaches did not exceed normal background levels (between 0.05μSv/Hr- 1.0 μSv/Hr). Furthermore, characteristic radionuclides released from this incident, specifically Cesium 134 and Cesium 134, were not detected. There was no sufficient difference between the radiation levels observed from the sand, water and different artifacts (logs, stones, and algae) Conclusion: It was concluded that the radiation levels along the beaches, Jericho, Spanish Banks and Sunset Beach do not present an additional risk to the public visiting this area as a result of this incident., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2017., Peer reviewed, Fukushima Daiichi, Cesium 134, Cesium 137, Gamma, Radiation, Vancouver beaches
Sampling of elementary school playground equipment for total coliforms and E. coli
Background and Purpose: Outbreaks of enteric diseases in schools and daycares are common. It is possible that these outbreaks could be propagated via fomites in school settings, such as playground equipment that is not regularly cleaned. Studies thus far have provided conflicting results on the level of contamination present on fomites in the school setting. This project is intended to assess the level of microbial contamination present on elementary school playground surfaces as a result of hand contact from school children. Methods: Two categories of elementary school playground equipment were sampled in this study; those that are likely to see regular hand contact from children and those that were not likely see hand regular hand contact from children. 30 surfaces of each category were swabbed and the media will be plated and incubated to enumerate total coliforms and E. coli. Results: The mean number of total coliforms on high hand contact surfaces was 0.2333 cfu/100cm2, while the mean number of total coliforms on low hand contact surfaces was 0.2667 cfu/100cm2. The t-test analysis of total coliform results produced a p-value of 0.5566. The mean number of E. coli on high hand contact surfaces was 1.1333 cfu/100cm2, while the mean number of E. coli on low hand contact surfaces was 4.9000 cfu/100cm2. The t-test analysis of E. coli results produced a p-value of 0.8019. Discussion: Neither results for total coliforms or E. coli indicated significantly different numbers on high or low hand contact surfaces. This indicates that these total coliform and E. coli may not be present as a result of hand contact. Conclusion: These results do not support the deposition of coliforms or E. coli on playground equipment as a result of hand contact. However, there is still concern due to the number of samples positive for E. coli. These results suggest the need for practices such as regular hand washing in the school setting after using the playground, regular cleaning of playground materials and EHO inspections of school grounds., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2016., Peer reviewed, Playgrounds, Schools, Surface sampling, Sanitation, Total coliforms, Escherichia coli, Abbotsford

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