This video demonstrates the spread plate technique, a common microbiological technique for enumeration of aerobic bacteria. This video shows inoculation from a broth culture, but an unknown liquid sample can also be used as your initial inoculum. Our laboratory uses metal spreaders, but glass spreaders can also be used in the same manner.
This video shows a set of expected results for after inoculation of broth, slants, and agar deeps, as well as streak plates, pour plates and spread plates. These would be the expected results from video 2, 4, 6 and 7.