Bioremediation has gained traction for its sustainable principles. Although, advancements in effectiveness are still needed to enable widespread application. This research has two major components. First, priming fungi could prove to be a useful tool to increase efficiency of white-rot fungi when used to bioremediate petroleum hydrocarbons contaminated soil. This study evaluated T. versicolor colonized in two substrates to test this theory. TPH was extracted from the soils using hexane shaking method, and measured on a CG-MS. The study results were not conclusive, and more research should be conducted to determine if priming white-rot fungi can increase the effectiveness of degradation of TPH in contaminated soils. Second, historical and unethical oil production in Ecuador has left an environmental and human health disaster. The goal of this study was to produce a high-level bioremediation plan that can be used and amended for site specific applications in Ecuador.