Environmental Public Health Journal 2016 | BCIT Institutional Repository

Environmental Public Health Journal 2016

Comparing the effectiveness between gel and foam hand sanitizers
Background: Hand sanitizers are commonly used as an alternative to washing hands with warm water and soap. There are a variety of different hand sanitizers including gel and foam and they are known to kill several bacteria. Many factors play a role in the effectiveness of hand sanitizers such as the alcohol concentration and the techniques used to apply hand sanitizers. Alcohol based hand sanitizers must have an alcohol concentration of 60 - 70% to be effective. There is currently no legislation regulating hand sanitizers and there is a lack of research focusing on differences between foam and gel hand sanitizers. This research study investigates effectiveness of gel compared to foam hand sanitizers by evaluating the prevalence of Escherichia coli (E.coli) on pigskins. Methods: To compare the hand sanitizers, microbiological sampling was completed. E.coli was introduced onto 65 pigskins. Five pigskins were used as a baseline to determine the average amount of Colony Forming Units (CFUs) of E.coli present prior to the application of hand sanitizers. One set of the 30 pigskins was applied with gel hand sanitizer, whereas the other 30 was applied with foam hand sanitizer. The pigskins were swabbed with QuickSnap swabs and plated onto 3M Petrifilms. The 65 petrifilms were incubated at 35oC for 48 hours. After incubation, the CFUs of E.coli present on the petrifilm were enumerated. The difference in CFUs was calculated to determine the reduction in E.coli and the overall effectiveness of hand sanitizers. Results: The data was analyzed by using the statistical software, NCSS. Statistical analysis showed that the findings were statistically significant and the null hypothesis (Ho: no difference in CFUs of E.coli between foam versus gel alcohol-based hand sanitizers) was rejected with a power of 0.9997 at p-value of 0.00000. This indicates that there is a difference in the ability to reduce E.coli between gel and foam hand sanitizers and gel sanitizers appeared to be more effective. Conclusion: These results indicate that there was a difference in the effectiveness between foam and gel hand sanitizers in reducing E.coli that was inoculated onto pigskins. However, consumers should be aware that hand sanitizers do not completely eliminate all pathogens. Though gel hand sanitizers are more effective, they should only be used when there are no other methods of keeping hands clean., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2016., Peer reviewed, E.coli, Escherichia coli, Foam, Gel, Hand Sanitizer, Pigskin, Public health
Evaluating the effectiveness of alcohol-based hand sanitizers compared to alcohol-free hand sanitizers
Background and Purpose: Hand washing is one of the most important critical control points in public premises in preventing the spread of bacteria and viruses. There is vast research on the effectiveness of alcohol-based hand sanitizers in killing germs. However, the efficacy of alcohol-free hand sanitizers lacks real-world evidence. With little to no guidelines in which one type of hand sanitizers may be more appropriate depending on the types of public premise such as food establishments, hospitals, work place, or schools, Environmental Health Officers(EHOs)/ Public Health Inspectors(PHIs) will need to educate the public and operators on the effectiveness of these hand sanitizers and their advantages and disadvantages. The purpose of the study was to compare the effectiveness of alcohol-based hand sanitizers and alcoholfree hand sanitizers by conducting statistical analyses of the reduction in mean E.coli counts. Methods: 60 pigskins were prepared (30 for alcohol-based hand sanitizers, 30 for alcohol-free hand sanitizers), which were inoculated with E. coli, then applied either alcohol-based hand sanitizers or alcoholfree hand sanitizers. After 48 hours of incubation for E.coli growth, E.coli was counted. The difference in mean E.coli counts before applying hand sanitizers and after hand sanitizers was calculated, then compared between the two hand sanitizers. Results: The mean E.coli reduction count (CFU) from alcohol-based hand sanitizers (30 samples) was 10.200; the median was 11; the standard deviation was 1.7889; the range was 5.0000. The mean E.coli reduction count (CFU) from alcohol-free hand sanitizers (30 samples) was 10.233; the median was 10.5; the standard deviation was 0.8976; the range was 3.0000. The statistical t-test resulted in p-value of 0.1034. Conclusion: There was no significant difference between the two types of hand sanitizers. Both the alcohol-based hand sanitizers and alcohol-free hand sanitizers effectively reduced the number of E.coli counts (CFU) by averages of 10.2000 (92.7% reduction) and 10.2333 (93.03% reduction) respectively. While the BC Centre for Disease Control recommends 60 percent alcohol hand sanitizers to prevent the spread of germs, this research showed that alcohol-free hand sanitizers with sulfactants, allantoin, and benzalkonium chloride (SAB) formula is just as effective in killing germs. Therefore, EHOs/PHIs can educate the public and operators on the advantages and disadvantages on the two types of hand sanitizers in preventing the spread germs during the flu season and give practical advice or guidance on which type of hand sanitizers would be most appropriate in restaurants for example., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2016., Peer reviewed, Alcohol-based hand sanitizers, Benzalkonium chloride, E. coli, Alcohol-free hand sanitizers
Evaluating the effectiveness of vinegar as a sanitizer
Objectives: Pathogens are introduced into foods, surfaces, and hands by our surrounding environment which includes soil, air, and fecal contamination. It can be due to improper handling, cleaning, washing or sanitizing. Sanitizers are applied to surfaces in order to kill all the vegetative cells of microbes. Health Canada regulates the types, uses and concentration of the sanitizers. These sanitizers are chemically formulated to kill microbes and hence there is a rising concern about toxicity associated with their use. People are moving away from regulated sanitizers to natural alternatives. This research project examined the efficacy of vinegar, one of the natural alternatives, as a sanitizer. Methods: 3M Quick Swabs were used to collect coliform samples from a plastic cutting board before and after inoculating it with coliform culture and subsequently cleaning it with vinegar. A one tail paired t-test was conducted to assess whether the coliform counts were reduced after cleaning with vinegar. Results: For all 30 samples there was a reduction in the number of coliforms when comparing before and after cleaning with vinegar. Results show that there is a significant difference in the mean numbers of coliforms before and after cleaning with vinegar; p <0.0001. Conclusion: These results indicate that undiluted vinegar when used for cleaning food contact surface significantly reduces the coliform counts but not to the safer levels for human exposure., Peer-reviewed article, Published., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2016., Peer reviewed, Sanitizer, Vinegar, Food contact surfaces, Alternatives, Efficacy