Environmental Public Health Journal 2014 | BCIT Institutional Repository

Environmental Public Health Journal 2014

Comparing the health risks of alfalfa sprouts and wheatgrass via detecting the presences of escherichia coli in their juices
Background: Past studies have analyzed the health risks associated with alfalfa sprout production and developed standard procedures to reduce foodborne illnesses. There have been no studies related to microgreen outbreaks, specifically wheatgrass. Wheatgrass has become a growing culinary trend and the potential health risks associated need to be evaluated. Alfalfa sprouts and wheatgrass both share the same initial growth production – pre-soak and germination. The only difference is the harvesting period. This paper evaluated the risks associated with alfalfa sprout production and compared it with wheatgrass production by contaminating both alfalfa sprouts and wheatgrass with E. coli The presences of E. coli in the plant’s juices were evaluated and compared. Method: Alfalfa sprouts and wheatgrass were grown in similar conditions, in hydroponic condition, with an additional wheatgrass in soil. The plants were grown and harvested according to its respective pre-soaking and harvesting period, as specified by the Canadian Food Inspection Agency. The plants were inoculated with Escherichia coli during the germination period, and then juiced to examine the presences of E. coli within its internal structure. The Hygiena systemSURE II luminometer was used to detect the presences of E. coli via the MicroSnap™ Enrichment and E. coli detection swabs. Results: The result showed that E. coli was present in both wheatgrass and alfalfa sprouts juice. The root systems of the food products were independent of each other. The types of growth medium used for wheatgrass were also independent of each other. Conclusion: The study found that growing microgreens should be treated similarly to sprout productions. Food facilities with wheatgrass production need to be aware of safe handling, production, and storage of wheatgrass to prevent foodborne illnesses., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Published., Peer reviewed, wheatgrass, microgreens, sprouts, foodborne illness, E. coli, root system, contamination
Determining the time required to disinfect a sponge contaminated with Escherichia coli, using a commercial microwave
Background: Disinfection and sanitation are important in areas where food is involved. Thorough cleaning is a necessity to prevent growth of harmful pathogens that could affect human health. Sponges used for cleaning can serve as a vehicle for cross-contamination on food preparation surfaces. There are various methods that could be used to disinfect contaminated sponges. The usage of a microwave is one suggested method. Objective: The purpose of this study was to determine if the Scotch-BriteTM Brand, cellulose sponges contaminated with E.coli (105 cfu/ml) could be disinfected using a microwave set at three timings (30 seconds, 1 minute, and 2 minutes). Methods: The Hygiena MicroSnap was used to detect the presence (or absence) of E.coli in sponges after microwave heating. The relative light units (RLU) indicated in the monitor determined whether there were any remaining coliforms in the sample after microwaving. Results: Statistical analysis was conducted using Microsoft Excel and NCSS. After heating sponges for 30 seconds, 100% of the samples detected no E.coli. After heating for 1 minute, 70% of the samples had no E.coli present. After heating for 2 minutes, 100% of the samples detected no E.coli. The p-value of 0.03567 concludes that the results were statistically significant at the 5% significance level. Discussion: The results of this study indicate that sponges contaminated with E.coli can be disinfected using microwave heating. EHOs, food establishment operators, and the general public can use this knowledge to re-use their old sponges and avoid further cross-contamination. Conclusions: The results indicate that microwave time is associated with the presence or absence of E.coli in a sponge. However, E.coli was present in 3 samples microwaved at 1 minute. This suggests further studies are required to confirm the findings of this study. In addition, further studies are required to determine what specific time is sufficient to completely eliminate the E.coli in a contaminated sponge., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Peer reviewed, E.coli, sponge, disinfection, microwave, MicroSnap, cross-contamination, EHO
Investigating the effectiveness of washing cantaloupe melon rind in preventing the transference of surface E. coli into melon flesh
Cantaloupe melon was the source of a lethal outbreak of Listeria in 2011. This research investigated whether washing a contaminated cantaloupe rind was sufficient in preventing the transferring of Escherichia coli. Hence, the null hypothesis for this study was that there is no association between washing a contaminated cantaloupe melon and the presence of the contamination in the flesh. In this study, 10 cantaloupes were used to produce a sample size of 20 per washed and unwashed treatments. Each of the samples was transferred to EC broth to determine the presence and absence of Escherichia coli (E. coli), the indicator organism that acted as the “outbreak contaminant.” The results showed 100% of the unwashed melons and 80% of the washed melons to have E. coli transferred into the flesh. A Chi Square analysis produced a p-value of 0.035. The study determined that there was a statistically significant association between washing a melon and the presence of E. coli in the melon flesh. The author recommends washing melon rind as a means to prevent foodborne illness caused by surface contaminants., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Published., Peer reviewed, Cantaloupe, melon, rind, flesh, outbreak, Escherichia coli, foodborne illness
Mechanically tenderized meat
Background: In 2012, mechanically tenderized meat raised public health concern when an E.Coli 0157:H7 outbreak was linked to the tenderization process. It was discovered that the machinery pushed the E.Coli from the surface of contaminated meat products such as steaks and roasts, into the interior, where it was able to survive the cooking process. Concerns were raised by Lorraine McIntyre and the BCCDC about this issue, and their desire to improve their knowledge base in order to adequately assess the risk. Methods: Data was gathered via a survey conducted electronically and by telephone. Questions were asked to determine the proportion of retail establishments that use their own tenderizing equipment. Questions also asked about other industry practices such as current sanitization and labeling practices. Results: The results of this study were that 24% of surveyed establishments mechanically tenderize their meat products. Of these establishments, 33% have a label that states the meat has been tenderized mechanically and 17% provide cooking instructions on this label. An association was found between mechanically tenderizing meat and establishment type, which suggests that grocery stores are more likely to mechanically tenderize than other establishments, such as restaurants. On the other hand, no association was found between operator experience and their level of knowledge regarding the risks of mechanical tenderization. Conclusions: Overall, this study has demonstrated the likelihood is high that consumers purchase and consume beef that has been mechanically tenderized at the retail level. The results from this study can be used to aid public health officials in quantifying the risk of mechanical tenderization at a retail level and aid in the development and implementation of new legislation such as mandatory labeling of all mechanically tenderized meat., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Published., Peer reviewed, mechanically tenderized meat (MTM), food safety, food retail establishment, survey, labeling
The risk of consuming MTGase-restructured steaks like intact steaks
Introduction: The use of transglutaminase to restructure loose pieces of meat into a fully intact piece of steak has been a concern for the public because of the potential internalization of contaminated surfaces into the aseptic center. The aim of this study was to examine if restructured steaks are safe to consume when cooked to medium rare, a common option with whole cut steaks Methods: Strips of beef were inoculated with E.coli to induce surface contamination. Steaks were restructured with transglutaminase and the altered meat. These steaks were then cooked alongside fully intact whole-cut steak samples. Each sample was then churned in a stomacher, and the resulting solution was used to detect for potential E.coli bacteria. Samples were then enriched and finally placed into the Hygiena Micro-snap Rapid Coliform and E.coli detection test to look for the presence of E.coli. Results: The Hygiena system showed that all transglutaminase restructured steaks possessed detectable levels of E.coli even after cooking to 55 degrees Celsius. On the other hand, no whole-cut steaks had traces of E.coli even when cooked to this same temperature. Conclusion: The results demonstrated that there is a substantial risk with restructured steaks and they should not be consumed undercooked. As well, proper labelling and guidelines should be developed to enable consumers to be better equipped in making decisions to consume properly consumed altered steaks., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Published., Peer reviewed, Transglutaminase, Meat glue, Steaks, Food safety, Escherichia coli