Environmental Public Health Journal 2014 | BCIT Institutional Repository

Environmental Public Health Journal 2014

Comparing the health risks of alfalfa sprouts and wheatgrass via detecting the presences of escherichia coli in their juices
Background: Past studies have analyzed the health risks associated with alfalfa sprout production and developed standard procedures to reduce foodborne illnesses. There have been no studies related to microgreen outbreaks, specifically wheatgrass. Wheatgrass has become a growing culinary trend and the potential health risks associated need to be evaluated. Alfalfa sprouts and wheatgrass both share the same initial growth production – pre-soak and germination. The only difference is the harvesting period. This paper evaluated the risks associated with alfalfa sprout production and compared it with wheatgrass production by contaminating both alfalfa sprouts and wheatgrass with E. coli The presences of E. coli in the plant’s juices were evaluated and compared. Method: Alfalfa sprouts and wheatgrass were grown in similar conditions, in hydroponic condition, with an additional wheatgrass in soil. The plants were grown and harvested according to its respective pre-soaking and harvesting period, as specified by the Canadian Food Inspection Agency. The plants were inoculated with Escherichia coli during the germination period, and then juiced to examine the presences of E. coli within its internal structure. The Hygiena systemSURE II luminometer was used to detect the presences of E. coli via the MicroSnap™ Enrichment and E. coli detection swabs. Results: The result showed that E. coli was present in both wheatgrass and alfalfa sprouts juice. The root systems of the food products were independent of each other. The types of growth medium used for wheatgrass were also independent of each other. Conclusion: The study found that growing microgreens should be treated similarly to sprout productions. Food facilities with wheatgrass production need to be aware of safe handling, production, and storage of wheatgrass to prevent foodborne illnesses., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Published., Peer reviewed, wheatgrass, microgreens, sprouts, foodborne illness, E. coli, root system, contamination
Fitness lunch bag
Background: Improper storage of food is one of the top ten food handling practices that cause a foodborne illness. This study assessed whether the Fitness Lunch Bag was able to keep precooked chicken breast cold at below 4°C (40°F) for 8 hours and to determine if the layers of the bag kept food consistent at the same temperature. Methods: Three SmartButtons, continuous temperature data logging devices, were inserted into three precooked and chilled chicken breasts. The three chicken breasts were placed within three trays inside the Fitness Lunch Bag that remained at room temperature for 8 hours. The internal temperatures were logged at one hour intervals. A total of 30 samples were collected. NCSS was used for statistical analysis of the data by regression/correlation and ANOVA. Results: The Fitness Lunch Bag was unable to hold cold food safely, at 4°C (40°F) and below for 8 hours. All three layers were found to exceed 4°C (40°F) within the first hour (p-value of 0.000 by 1 sample t-test). In addition, all three layers of the Fitness Lunch Bag were found to not be equivalent in retaining a consistent temperature throughout the bag (p-value of 0.000000 < 0.05 by regression analysis at the 8 hour mark). Layer 2 was the best at retaining the lowest temperature for the chicken breasts, followed by layer 1. Moreover, layer 3 was found to display the warmest temperatures out of the three layers. Conclusion: The results of the study show that the Fitness Lunch Bag does not have the ability to keep cold foods out of the danger zone, 4-60°C (40 – 140°F), for 8 hours. In addition, the bag was not capable of keeping cold foods safe at the 1 hour mark. Caution should be used with any type of lunch bag, insulated and/or frozen gel packed, especially with the potentially hazardous foods., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Published., Peer reviewed, Peer-reviewed article, lunch bag, foodborne illness, temperature, temperature abuse, fitness
Investigating the effectiveness of washing cantaloupe melon rind in preventing the transference of surface E. coli into melon flesh
Cantaloupe melon was the source of a lethal outbreak of Listeria in 2011. This research investigated whether washing a contaminated cantaloupe rind was sufficient in preventing the transferring of Escherichia coli. Hence, the null hypothesis for this study was that there is no association between washing a contaminated cantaloupe melon and the presence of the contamination in the flesh. In this study, 10 cantaloupes were used to produce a sample size of 20 per washed and unwashed treatments. Each of the samples was transferred to EC broth to determine the presence and absence of Escherichia coli (E. coli), the indicator organism that acted as the “outbreak contaminant.” The results showed 100% of the unwashed melons and 80% of the washed melons to have E. coli transferred into the flesh. A Chi Square analysis produced a p-value of 0.035. The study determined that there was a statistically significant association between washing a melon and the presence of E. coli in the melon flesh. The author recommends washing melon rind as a means to prevent foodborne illness caused by surface contaminants., Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2014., Peer-reviewed article, Published., Peer reviewed, Cantaloupe, melon, rind, flesh, outbreak, Escherichia coli, foodborne illness